KMB (Kings Medium B Agar)
20g Proteose Peptone No. 3 (Difco)
1.5g K2HPO4
0.738g MgSO4 (Anhydrous) or 1.5g MgSO4*7H2O
10ml Glycerol
990ml ddH2O
15g Bacto Agar
Mix Proteose Peptone, K2HPO4, MgSO4, and ddH2O till in solution. Add glycerol with a pipette. Rinse the pipette in the solution to remove residual glycerol from the inside of the pipette. Adjust pH to 7.4. Add agar and autoclave.
金氏培養基(甲)
蛋白腖20g，無水氯化鎂1.4g，無水硫酸鉀10g，瓊脂15g，甘油10ml，蒸餾水1L。
將上述成分加熱、溶解，調pH至7.2，分裝試管，118~121℃滅菌15min，制成斜面和高層備用。