FAM?FITC

FAM?FITC?

?

FAM?FITC?Lys?Orn?Dap?DabN

?: FITC?R-N=C=S?:

R-N=C=S? + NH2 ? R-NH-C=S + H2O

FAM:

COOH + NH2 ? CO-NH- peptide bond

FITC?pH>8FAM?HATU?HBTU

FITCN?LinkerAhx?6-PEG2?Beta-AlaEdman?Linker?

FITC?Linker?

FAMLinker

FAMFITC